APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 40% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BLNK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240510
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
B cell linker , AGM4 , BASH , BLNK-S , LY57 , SLP-65 , SLP65 , bca
Cellular Localization
Cytoplasm , Cell membrane
Background
This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-BLNK antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104614](/templates/default/js/lightbox/images/loading.gif "Anti-BLNK antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104614")
|
GTX104614 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using B-cell linker protein(GTX104614) antibody at 1:200 dilution.
|
|
|
GTX104614 WB Image
Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX104614 diluted at 1:500
|
REFERENCE
There are currently no references for BLNK antibody [C3], C-term (GTX104614). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BLNK antibody [C3], C-term (GTX104614). Be the first to share your experience with this product.
![Anti-BLNK antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104614](/upload/website/prouct_img/small/GTX104614/GTX104614_IFA_w_23060120_726.webp "Anti-BLNK antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104614")
![Anti-BLNK antibody [C3], C-term used in Western Blot (WB). GTX104614](/upload/website/prouct_img/small/GTX104614/GTX104614_WB_w_23060120_404.webp "Anti-BLNK antibody [C3], C-term used in Western Blot (WB). GTX104614")
![Anti-BLNK antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104614](/upload/website/prouct_img/normal/GTX104614/GTX104614_IFA_w_23060120_726.webp "Anti-BLNK antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104614")
![Anti-BLNK antibody [C3], C-term used in Western Blot (WB). GTX104614](/upload/website/prouct_img/normal/GTX104614/GTX104614_WB_w_23060120_404.webp "Anti-BLNK antibody [C3], C-term used in Western Blot (WB). GTX104614")