GeneTex
United States (US)

BLNK antibody

BLNK-antibody__GTX15008-1_18121409_553.jpg

Cat No. GTX15008

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

WB: Use at an assay dependent dilution. Predicted molecular weight: 54.7 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

50 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fusion protein representing amino acids 171-356 of human BLNK.

Purification

>95% by SDS-PAGE
Purified from rabbit antisera by precipitation methods.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B Cell Linker,Agm4,Bash,Blnk-S,Ly57,Slp-65,Slp65,Bca,Blnk

Background

BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.

Database

Research Area

DATA IMAGES
BLNK-antibody__GTX15008-1_18121409_553.jpg

GTX15008 WB Image

Western Blot analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using polyclonal anti-mouse SLP65 / BLNK antibody (GTX15008).

Package List Price ($)
$ 319