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BLZF1 antibody

Anti-BLZF1 antibody used in Western Blot (WB). GTX116434
Anti-BLZF1 antibody used in Western Blot (WB). GTX116434
Anti-BLZF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116434
Anti-BLZF1 antibody used in Western Blot (WB). GTX116434

Cat. No. GTX116434

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all BLZF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

NIH-3T3 , Huh7 , Hep3B , HepG2 , BLZF-transfected 293T

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BLZF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168554

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basic leucine zipper nuclear factor 1 , GOLGIN-45 , JEM-1 , JEM-1s , JEM1

Cellular Localization

Golgi apparatus lumen , Isoform 1: Nucleus , Isoform 2: Cytoplasm

Background

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.

Database

Research Area

DATA IMAGES

Anti-BLZF1 antibody used in Western Blot (WB). GTX116434

GTX116434 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX116434 diluted at 1:1000

Anti-BLZF1 antibody used in Western Blot (WB). GTX116434

GTX116434 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BLZF1 antibody (GTX116434) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BLZF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116434

GTX116434 ICC/IF Image

BLZF1 antibody detects BLZF1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BLZF1 protein stained by BLZF1 antibody (GTX116434) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-BLZF1 antibody used in Western Blot (WB). GTX116434

GTX116434 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BLZF1 antibody (GTX116434) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

N?chel J et al. Mol Cell 2021; 81 (16) : 3275-3293.e12 An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169