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BMAL1 antibody [3G9]

Overexpression
Anti-BMAL1 antibody [3G9] used in Flow cytometry (FACS). GTX84851
Anti-BMAL1 antibody [3G9] used in Western Blot (WB). GTX84851
Anti-BMAL1 antibody [3G9] used in Western Blot (WB). GTX84851
Anti-BMAL1 antibody [3G9] used in Western Blot (WB). GTX84851
Anti-BMAL1 antibody [3G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84851
Anti-BMAL1 antibody [3G9] used in Flow cytometry (FACS). GTX84851

Cat. No. GTX84851

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G9

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($519)
See all BMAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ARNTL (NP_001169) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10727812

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aryl hydrocarbon receptor nuclear translocator like , BMAL1 , BMAL1c , JAP3 , MOP3 , PASD3 , TIC , bHLHe5

Cellular Localization

Nucleus,Cytoplasm

Background

ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.

Database

Research Area

DATA IMAGES

Anti-BMAL1 antibody [3G9] used in Flow cytometry (FACS). GTX84851

GTX84851 FACS Image

FACS analysis of Jurkat cells using GTX84851 BMAL1 antibody [3G9].
Red : Primary antibody
Blue : Negative control antibody

Anti-BMAL1 antibody [3G9] used in Western Blot (WB). GTX84851

GTX84851 WB Image

WB analysis of various samples using GTX84851 BMAL1 antibody [3G9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-BMAL1 antibody [3G9] used in Western Blot (WB). GTX84851

GTX84851 WB Image

Overexpression

WB analysis of HEK293T cells transfected with BMAL1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84851 BMAL1 antibody [3G9].
Loading : 5 ug per lane

Anti-BMAL1 antibody [3G9] used in Western Blot (WB). GTX84851

GTX84851 WB Image

WB analysis of various cell lines using GTX84851 BMAL1 antibody [3G9].
Loading : 35 ug per lane

Anti-BMAL1 antibody [3G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84851

GTX84851 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with BMAL1 plasmid using GTX84851 BMAL1 antibody [3G9].

Anti-BMAL1 antibody [3G9] used in Flow cytometry (FACS). GTX84851

GTX84851 FACS Image

FACS analysis of HEK293T cells transfected with either BMAL1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84851 BMAL1 antibody [3G9].

REFERENCE

Application Reference

Li T et al. Medicine & Healthcare 2017; 1 : 4 Effects of * Sleep Deprivation on Liver Circadian Clock Gene Expression and Oxidative Stress in Rats.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519