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BMAL1 antibody [HL2456]

Recombinant antibody KO/KD Orthogonal
Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774
Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774
Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774
Anti-BMAL1 antibody [HL2456] used in IHC (Paraffin sections) (IHC-P). GTX638774
Anti-BMAL1 antibody [HL2456] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638774
Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774

Cat. No. GTX638774

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2456

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BMAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human BMAL1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aryl hydrocarbon receptor nuclear translocator like , BMAL1 , BMAL1c , JAP3 , MOP3 , PASD3 , TIC , bHLHe5

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774

GTX638774 WB Image

KO/KD

Non-transfected (–) and transfected (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774

GTX638774 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774

GTX638774 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-BMAL1 antibody [HL2456] used in IHC (Paraffin sections) (IHC-P). GTX638774

GTX638774 IHC-P Image

BMAL1 antibody [HL2456] detects BMAL1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
BMAL1 stained by BMAL1 antibody [HL2456] (GTX638774) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BMAL1 antibody [HL2456] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638774

GTX638774 ICC/IF Image

BMAL1 antibody [HL2456] detects BMAL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT-116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BMAL1 stained by BMAL1 antibody [HL2456] (GTX638774) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-BMAL1 antibody [HL2456] used in Western Blot (WB). GTX638774

GTX638774 WB Image

Orthogonal

Untreated and treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations