Non-transfected (–) and transfected (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated and treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BMAL1 antibody [HL2456] (GTX638774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

BMAL1 antibody [HL2456] detects BMAL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT-116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BMAL1 stained by BMAL1 antibody [HL2456] (GTX638774) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

BMAL1 antibody [HL2456] detects BMAL1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
BMAL1 stained by BMAL1 antibody [HL2456] (GTX638774) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min