GeneTex
United States (US)

BMAL1 antibody, Internal

GTX88473_2295_WB_18122102_953.jpg

Cat No. GTX88473

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88473-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_001025444.1; NP_001025443.1). Reported variants represent identical protein (NP_001025443.1; NP_001169.3).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence REKITTNCYKFKIKD, from the internal region of the protein sequence according to NP_001169.3; NP_001025444.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PASD3 Antibody , TIC Antibody , BHLHE5 Antibody , BMAL1C Antibody , JAP3 Antibody , MOP3 Antibody , ARNTL Antibody

Background

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Show More +

Database

Research Area

DATA IMAGES
GTX88473_2295_WB_18122102_953.jpg

GTX88473 WB Image

WB analysis of human brain (cerebellum) lysate using GTX88473 BMAL1 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer