GeneTex
United States (US)

BMAL1 antibody [N1N3]

GTX105060_39876_IFA_19011506_599.jpg
GTX105060_39876_WB_M_brain_19011506_321.jpg
GTX105060_WB_19011506_812.jpg

Cat No. GTX105060

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

BMAL1 tansfected 293T cell , mouse brain

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BMAL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BMAL1 antibody, BMAL1c antibody, JAP3 antibody, MGC47515 antibody, MOP3 antibody, PASD3 antibody, TIC antibody, bHLHe5 antibody, ARNTL antibody, basic-helix-loop-helix-PAS orphan MOP3 antibody, bHLH-PAS protein JAP3 antibody, PAS domain-containing protein 3 antibody, basic-helix-loop-helix-PAS protein MOP3 antibody, brain and muscle ARNT-like 1 antibody, "ARNT-like protein 1, brain and muscle antibody", aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody, member of PAS protein 3 antibody, class E basic helix-loop-helix protein 5 antibody, member of PAS superfamily 3 antibody, aryl hydrocarbon receptor nuclear translocator-like antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105060_39876_IFA_19011506_599.jpg

GTX105060 ICC/IF Image

MOP3 antibody [N1N3] detects MOP3 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MOP3 protein stained by MOP3 antibody [N1N3] (GTX105060) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX105060_39876_WB_M_brain_19011506_321.jpg

GTX105060 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105060 diluted at 1:1000

GTX105060_WB_19011506_812.jpg

GTX105060 WB Image

Western Blot analysis of ARNTL expression in transfected 293T cell line by ARNTL polyclonal antibody.

A: Non-transfected lysate.

B: ARNTL transfected lysate.

7.5% SDS PAGE

GTX105060 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169