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BMAL1 antibody [N1N3]

Orthogonal Overexpression
Anti-BMAL1 antibody [N1N3] used in Western Blot (WB). GTX105060
Anti-BMAL1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105060
Anti-BMAL1 antibody [N1N3] used in Western Blot (WB). GTX105060
Anti-BMAL1 antibody [N1N3] used in Western Blot (WB). GTX105060

Cat No. GTX105060

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all BMAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

BMAL1 tansfected 293T cell , mouse brain

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BMAL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aryl hydrocarbon receptor nuclear translocator like , BMAL1 , BMAL1c , JAP3 , MOP3 , PASD3 , TIC , bHLHe5

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BMAL1 antibody [N1N3] used in Western Blot (WB). GTX105060

GTX105060 WB Image

Orthogonal

Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] (GTX105060) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 16109848, 28803917

Anti-BMAL1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105060

GTX105060 ICC/IF Image

BMAL1 antibody [N1N3] detects BMAL1 protein at nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BMAL1 protein stained by BMAL1 antibody [N1N3] (GTX105060) diluted at 1:500. Blue: Hoechst 33342 staining.

Anti-BMAL1 antibody [N1N3] used in Western Blot (WB). GTX105060

GTX105060 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105060 diluted at 1:1000

Anti-BMAL1 antibody [N1N3] used in Western Blot (WB). GTX105060

GTX105060 WB Image

Overexpression

Western Blot analysis of BMAL1 expression in transfected 293T cell line by BMAL1 polyclonal antibody. A: Non-transfected lysate. B: BMAL1 transfected lysate. 7.5% SDS PAGE GTX105060 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169