APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
BMAL1 tansfected 293T cell , mouse brain
Predict Reactivity
Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.65mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human BMAL1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BMAL1 antibody, BMAL1c antibody, JAP3 antibody, MGC47515 antibody, MOP3 antibody, PASD3 antibody, TIC antibody, bHLHe5 antibody, ARNTL antibody, basic-helix-loop-helix-PAS orphan MOP3 antibody, bHLH-PAS protein JAP3 antibody, PAS domain-containing protein 3 antibody, basic-helix-loop-helix-PAS protein MOP3 antibody, brain and muscle ARNT-like 1 antibody, "ARNT-like protein 1, brain and muscle antibody", aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody, member of PAS protein 3 antibody, class E basic helix-loop-helix protein 5 antibody, member of PAS superfamily 3 antibody, aryl hydrocarbon receptor nuclear translocator-like antibody
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105060 ICC/IF Image
MOP3 antibody [N1N3] detects MOP3 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MOP3 protein stained by MOP3 antibody [N1N3] (GTX105060) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX105060 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105060 diluted at 1:1000
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GTX105060 WB Image
Western Blot analysis of ARNTL expression in transfected 293T cell line by ARNTL polyclonal antibody.
A: Non-transfected lysate.
B: ARNTL transfected lysate.
7.5% SDS PAGE
GTX105060 diluted at 1:500
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