APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , ARNTL transfected 293T cell
Predict Reactivity
Rat, Sheep(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.65 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human BMAL1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949686
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
aryl hydrocarbon receptor nuclear translocator like , BMAL1 , BMAL1c , JAP3 , MOP3 , PASD3 , TIC , bHLHe5
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105060 WB Image
Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] (GTX105060) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 16109848, 28803917
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GTX105060 ICC/IF Image
BMAL1 antibody [N1N3] detects BMAL1 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BMAL1 protein stained by BMAL1 antibody [N1N3] (GTX105060) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX105060 WB Image
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX105060 diluted at 1:1000
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GTX105060 WB Image
Western Blot analysis of BMAL1 expression in transfected 293T cell line by BMAL1 polyclonal antibody.
A: Non-transfected lysate.
B: BMAL1 transfected lysate.
7.5% SDS PAGE
GTX105060 diluted at 1:500
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REFERENCE
There are currently no references for BMAL1 antibody [N1N3] (GTX105060). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BMAL1 antibody [N1N3] (GTX105060). Be the first to share your experience with this product.