GeneTex

  • Country / Location Selection

United States (US)

BMAL1 antibody

Orthogonal
Anti-BMAL1 antibody used in Western Blot (WB). GTX50643
Anti-BMAL1 antibody used in Western Blot (WB). GTX50643
Anti-BMAL1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX50643

Cat. No. GTX50643

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all BMAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.569~573 (S-S-S-I-L) derived from human BMAL1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169224

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aryl hydrocarbon receptor nuclear translocator like , BMAL1 , BMAL1c , JAP3 , MOP3 , PASD3 , TIC , bHLHe5

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-BMAL1 antibody used in Western Blot (WB). GTX50643

GTX50643 WB Image

WB analysis of extracts from HL-60, THP-1, 3T3, HepG2, and MCF-7 cells using GTX50643 BMAL1 antibody.

Anti-BMAL1 antibody used in Western Blot (WB). GTX50643

GTX50643 WB Image

WB analysis of extracts from mouse brain tissue using GTX50643 BMAL1 antibody.

Anti-BMAL1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX50643

GTX50643 IHC-Fr Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 25822259

REFERENCE

Application Reference

Hou J et al. Behav Brain Res 2019; 364 : 167-176 rem sleep deprivation-induced circadian clock gene abnormalities participate in hippocampal-dependent memory impairment by enhancing inflammation in rats undergoing * inhalation.
Application : WB
Reactivity : Rat
Hardman JA et al. PLoS One 2015; 10 (3) : e0121878 Thyroxine differentially modulates the peripheral clock: lessons from the human hair follicle.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for BMAL1 antibody (GTX50643). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399