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BMF antibody

Anti-BMF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29653
Anti-BMF antibody used in Western Blot (WB). GTX29653
Anti-BMF antibody used in Western Blot (WB). GTX29653

Cat. No. GTX29653

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($479)
See all BMF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2.5-5μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Product Note

This antibody recognizes an epitope at the C-terminus of human Bmf (Bcl-2-Modifying Factor), a novel BH3-only protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 171- 184 of human Bmf (accession no. NP_277038).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BMF , Bcl2 modifying factor

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BMF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29653

GTX29653 ICC/IF Image

Immunofluorescent staining of Bmf in human kidney tissue with Bmf antibody (GTX29653) at 10μg/ml.

Anti-BMF antibody used in Western Blot (WB). GTX29653

GTX29653 WB Image

WB analysis of HepG2 cell lysate using GTX29653 BMF antibody.
Dilution : 2.5μg/ml (Lane A) and 5μg/ml (Lane B)

Anti-BMF antibody used in Western Blot (WB). GTX29653

GTX29653 WB Image

Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with Anti-Bmf (Cat. No. GTX29653) at 2 mg /mL.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 479