GeneTex
United States (US)

BMF antibody

BMF-antibody_ICCIF_GTX29655-1_18121409_736.jpg
BMF-antibody_WB_GTX29655-1_18121409_298.jpg

Cat No. GTX29655

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

This antibody can be used for detection of Bmf by Western blot at 2 μg/ml. HepG2 or 293 cell lysate can be used as a positive control and a band at approximately 25 kDa can be detected.

Calculated MW

21 kDa. ( Note )

Positive Control

HepG2 or 293 cell lysate.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: EPSQCVEELEDDV, corresponding to amino acids 2-14 of Human Bmf.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Modifying Factor,Bmf

Background

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor) (1). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2). Bmf is constitutively expressed in many tissues (1,2).

Database

Research Area

DATA IMAGES
BMF-antibody_ICCIF_GTX29655-1_18121409_736.jpg

GTX29655 ICC/IF Image

Immunocytochemical staining of Bmf in HeLa cells with Bmf antibody (GTX29655)at 10μg/ml.

BMF-antibody_WB_GTX29655-1_18121409_298.jpg

GTX29655 WB Image

Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with Bmf antibody (GTX29655) at 2μg /ml.

Package List Price ($)
$ 329