*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HepG2 conditioned medium , NIH-3T3 , JC
This antibody is able to recognize full length BMP4 protein (~55kDa) and N-terminal propeptide BMP4 protein (~40kDa) but unable to detect mature form.
Rat, Rabbit, Sheep, Bovine, Dog, Pig(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.36 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human BMP4. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
bone morphogenetic protein 4 , BMP2B , BMP2B1 , MCOPS6 , OFC11 , ZYME
Secreted , extracellular space , extracellular matrix
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq]
GTX100874 WB Image
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100874) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX100874 IHC-P Image
Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using BMP4(GTX100874) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX100874 WB Image
Sample (30 μg of whole cell lysate)
10% SDS PAGE
GTX100874 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX100874 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using BMP4(GTX100874) antibody at 1:100 dilution.