GeneTex
United States (US)

BMP4 antibody

Orthogonal
GTX100874_42998_20171124_WB_Fraction_19011506_269.jpg
GTX100874_40051_IHC_19011506_965.jpg
GTX100874_40051_WB_M_19011506_945.jpg
GTX100874_40051_IFA_19011506_449.jpg

Cat No. GTX100874

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 conditioned medium , NIH-3T3 , JC

Specificity/Sensitivity

This antibody is able to recognize full length BMP4 protein (~55kDa) and N-terminal propeptide BMP4 protein (~40kDa) but unable to detect mature form.

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BMP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BMP2B antibody, BMP2B1 antibody, MCOPS6 antibody, OFC11 antibody, ZYME antibody, BMP4 antibody, BMP-2B antibody, bone morphogenetic protein 4 antibody, bone morphogenetic protein 2B antibody, BMP-4 antibody

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100874_42998_20171124_WB_Fraction_19011506_269.jpg

GTX100874 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100874) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100874_40051_IHC_19011506_965.jpg

GTX100874 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using BMP4(GTX100874) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100874_40051_WB_M_19011506_945.jpg

GTX100874 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX100874 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100874_40051_IFA_19011506_449.jpg

GTX100874 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using BMP4(GTX100874) antibody at 1:100 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169