*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HepG2 conditioned medium , BMP4-transfected 293T
Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.88 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the C-terminus region of human BMP4. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Bone Morphogenetic Protein 4,Bmp2B,Bmp2B1,Mcops6,Ofc11,Zyme,Bmp4
Secreted , extracellular space , extracellular matrix
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq]
GTX100875 WB Image
293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX100875 WB Image
HepG2 conditioned medium (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.