GeneTex
United States (US)

BMP4 antibody

GTX100875_43257_20180629_WB_B_19011506_699.jpg
GTX100875_43257_20180629_WB_Fraction_19011506_642.jpg

Cat No. GTX100875

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 conditioned medium , BMP4-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BMP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bone Morphogenetic Protein 4,Bmp2B,Bmp2B1,Mcops6,Ofc11,Zyme,Bmp4

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq]

Research Area

DATA IMAGES
GTX100875_43257_20180629_WB_B_19011506_699.jpg

GTX100875 WB Image

293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100875_43257_20180629_WB_Fraction_19011506_642.jpg

GTX100875 WB Image

HepG2 conditioned medium (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169