BMP4 antibody

Name: BMP4 antibody
Brand: GeneTex
SKU: GTX100875
Price: 399.00 USD

Anti-BMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX100875

Anti-BMP4 antibody used in Western Blot (WB). GTX100875

Cat. No. GTX100875

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all BMP4 products

View product citations for antibody GTX100875 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
IHC-P	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

BMP4-transfected 293T

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BMP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949750

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bone morphogenetic protein 4 , BMP2B , BMP2B1 , MCOPS6 , OFC11 , ZYME

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers. [provided by RefSeq, Jul 2016]

Database

Gene ID: 652 BMP4
UniProt: P12644 BMP4

Research Area

DATA IMAGES

GTX100875 IHC-P Image

BMP4 antibody detects BMP4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat smooth muscle.
BMP4 stained by BMP4 antibody (GTX100875) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100875 IHC-P Image

BMP4 antibody detects BMP4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
BMP4 stained by BMP4 antibody (GTX100875) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100875 WB Image

HepG2 conditioned medium (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100875 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.