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BMP4 antibody

Anti-BMP4 antibody used in Western Blot (WB). GTX100875
Anti-BMP4 antibody used in Western Blot (WB). GTX100875

Cat No. GTX100875

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC

Reactivity

Human
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 conditioned medium , BMP4-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BMP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

bone morphogenetic protein 4 , BMP2B , BMP2B1 , MCOPS6 , OFC11 , ZYME

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-BMP4 antibody used in Western Blot (WB). GTX100875

GTX100875 WB Image

293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BMP4 antibody used in Western Blot (WB). GTX100875

GTX100875 WB Image

HepG2 conditioned medium (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169