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BMP5 antibody

Anti-BMP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03273
Anti-BMP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03273
Anti-BMP5 antibody used in Western Blot (WB). GTX03273
Anti-BMP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03273
Anti-BMP5 antibody used in Flow cytometry (FACS). GTX03273

Cat. No. GTX03273

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all BMP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cells
ELISA 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BMP-5 (332-365aa HQDSSRMSSVGDYNTSEQKQACKKHELYVSFRDL), different from the related mouse sequence by three amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bone morphogenetic protein 5

Cellular Localization

Secreted

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Polymorphisms in this gene may be associated with osteoarthritis in human patients. This gene is differentially regulated in multiple human cancers. This gene encodes distinct protein isoforms that may be similarly proteolytically processed. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-BMP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03273

GTX03273 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX03273 BMP5 antibody.

Anti-BMP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03273

GTX03273 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX03273 BMP5 antibody.

Anti-BMP5 antibody used in Western Blot (WB). GTX03273

GTX03273 WB Image

WB analysis of various sample lysates using GTX03273 BMP5 antibody.
Lane 1 : Rat Liver tissue lysate (50μg)
Lane 2 : Mouse Liver tissue lysate (50μg)
Lane 3 : A549 cell lysate (40μg)
Dilution : 0.5μg/ml

Anti-BMP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03273

GTX03273 IHC-P Image

IHC-P analysis of rat lung tissue using GTX03273 BMP5 antibody.

Anti-BMP5 antibody used in Flow cytometry (FACS). GTX03273

GTX03273 FACS Image

FACS analysis of U2OS cells using GTX03273 BMP5 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1μg/1x10⁶ cells

REFERENCE

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REVIEW

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Package List Price ($)
$ 429