Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP7 antibody (GTX132085) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BMP7 antibody (GTX132085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BMP7 antibody (GTX132085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

BMP7 antibody detects BMP7 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BMP7 antibody (GTX132085) diluted at 1:3000.