BMPR1B antibody [N3C3]

Name: BMPR1B antibody [N3C3]
Brand: GeneTex
SKU: GTX102453
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-BMPR1B antibody [N3C3] used in Western Blot (WB). GTX102453

Anti-BMPR1B antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102453

Anti-BMPR1B antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX102453

Cat. No. GTX102453

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat, Bovine
Package	100 μl ($399), 25 μl ($169)

See all BMPR1B products

View product citations for antibody GTX102453 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , human BMPR1B-transfected 293T cells , Neuro2A , C8D30 , NIH-3T3 , Rat2

Predict Reactivity

Sheep, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human BMPR1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615371

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bone morphogenetic protein receptor type 1B , ALK-6 , ALK6 , AMDD , BDA1D , BDA2 , CDw293

Cellular Localization

Membrane

Background

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. [provided by RefSeq]

Database

Gene ID: 658 BMPR1B
UniProt: O00238 BMPR1B

Research Area

DATA IMAGES

GTX102453 WB Image

BMPR1B antibody detects BMPR1B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody (GTX102453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102453 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody [N3C3] (GTX102453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102453 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody [N3C3] (GTX102453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102453 IHC-P Image

BMPR1B antibody [N3C3] detects BMPR1B protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
BMPR1B antibody [N3C3] (GTX102453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102453 WB Image

BMPR1B antibody detects BMPR1B protein by western blot analysis. Non-transfected (-) and BMPR1B-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody (GTX102453) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102453 IHC-Fr Image

BMPR1B antibody [N3C3] detects BMPR1B protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: BMPR1B protein stained by BMPR1B antibody [N3C3] (GTX102453) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yoshino H et al. Oncol Rep 2018; 39 (1) : 173-181 Downregulation of microRNA-1274a induces cell apoptosis through regulation of BMPR1B in clear cell renal cell carcinoma.

Application : IHC-P

Reactivity : Human

Chung D et al. Mol Cell Endocrinol 2015; 400 : 48-60 Estrogen mediated epithelial proliferation in the uterus is directed by stromal Fgf10 and Bmp8a.

Application : WB

Reactivity : Mouse

Daesuk Chung et al. Molecular and Cellular Endocrinology 2014; Estrogen mediated epithelial proliferation in the uterus is directed by stromal Fgf10 and Bmp8a

Application : WB

Reactivity : Mouse

Gasperin BG et al. Anim Reprod Sci 2014; 144 (43163) : 26512 Expression of receptors for BMP15 is differentially regulated in dominant and subordinate follicles during follicle deviation in cattle.

Application : WB

Reactivity : Bovine

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