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BMPR1B antibody [N3C3]

Anti-BMPR1B antibody [N3C3] used in Western Blot (WB). GTX102453
Anti-BMPR1B antibody [N3C3] used in Western Blot (WB). GTX102453
Anti-BMPR1B antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102453
Anti-BMPR1B antibody [N3C3] used in Western Blot (WB). GTX102453
Anti-BMPR1B antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX102453
Anti-BMPR1B antibody [N3C3] used in Western Blot (WB). GTX102453

Cat No. GTX102453

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Bovine
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , Neuro2A , C8D30 , NIH-3T3 , human BMPR1B-transfected 293T , Rat2

Predict Reactivity

Sheep, Dog, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BMPR1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

bone morphogenetic protein receptor type 1B , ALK-6 , ALK6 , AMDD , BDA1D , BDA2 , CDw293

Cellular Localization

Membrane

Background

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-BMPR1B antibody [N3C3] used in Western Blot (WB). GTX102453

GTX102453 WB Image

BMPR1B antibody detects BMPR1B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody (GTX102453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BMPR1B antibody [N3C3] used in Western Blot (WB). GTX102453

GTX102453 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody [N3C3] (GTX102453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BMPR1B antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102453

GTX102453 IHC-P Image

BMPR1B antibody [N3C3] detects BMPR1B protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
BMPR1B antibody [N3C3] (GTX102453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BMPR1B antibody [N3C3] used in Western Blot (WB). GTX102453

GTX102453 WB Image

BMPR1B antibody detects BMPR1B protein by western blot analysis. Non-transfected (-) and BMPR1B-transfected (+, ) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody (GTX102453) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BMPR1B antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX102453

GTX102453 IHC-Fr Image

BMPR1B antibody [N3C3] detects BMPR1B protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: BMPR1B protein stained by BMPR1B antibody [N3C3] (GTX102453) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BMPR1B antibody [N3C3] used in Western Blot (WB). GTX102453

GTX102453 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody [N3C3] (GTX102453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169