GeneTex
United States (US)

BMPR1B antibody [N3C3]

Overexpression
GTX102453_42137_20150806_WB_19011506_192.jpg
GTX102453_42137_20160811_WB_M_19011506_599.jpg
GTX102453_41045_IHC_19011506_102.jpg
GTX102453_41773_20150709_WB_B_19011506_566.jpg
GTX102453_42137_20160830_IHC-Fr_19011506_299.jpg
GTX102453_42137_20160811_WB_R_19011506_800.jpg

Cat No. GTX102453

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , Neuro2A , C8D30 , NIH-3T3 , human BMPR1B-transfected 293T , Rat2

Predict Reactivity

Sheep, Dog, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BMPR1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALK-6 antibody, ALK6 antibody, CDw293 antibody, BMPR1B antibody, BMPR-1B antibody, serine/threonine receptor kinase antibody, BMP type-1B receptor antibody, bone morphogenetic protein receptor type-1B antibody, activin receptor-like kinase 6 antibody, "bone morphogenetic protein receptor, type IB antibody"

Cellular Localization

Membrane

Background

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102453_42137_20150806_WB_19011506_192.jpg

GTX102453 WB Image

BMPR1B antibody detects BMPR1B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody (GTX102453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102453_42137_20160811_WB_M_19011506_599.jpg

GTX102453 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody [N3C3] (GTX102453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102453_41045_IHC_19011506_102.jpg

GTX102453 IHC-P Image

BMPR1B antibody [N3C3] detects BMPR1B protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
BMPR1B antibody [N3C3] (GTX102453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102453_41773_20150709_WB_B_19011506_566.jpg

GTX102453 WB Image

Overexpression

BMPR1B antibody detects BMPR1B protein by western blot analysis. Non-transfected (-) and BMPR1B-transfected (+, ) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody (GTX102453) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102453_42137_20160830_IHC-Fr_19011506_299.jpg

GTX102453 IHC-Fr Image

BMPR1B antibody [N3C3] detects BMPR1B protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: BMPR1B protein stained by BMPR1B antibody [N3C3] (GTX102453) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102453_42137_20160811_WB_R_19011506_800.jpg

GTX102453 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody [N3C3] (GTX102453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169