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BNIP3 antibody

Anti-BNIP3 antibody used in Western Blot (WB). GTX64429
Anti-BNIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64429
Anti-BNIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64429
Anti-BNIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64429
Anti-BNIP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64429

Cat. No. GTX64429

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all BNIP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human BNIP3 (NP_004043.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 interacting protein 3 , NIP3

Cellular Localization

Mitochondrion

Background

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-BNIP3 antibody used in Western Blot (WB). GTX64429

GTX64429 WB Image

WB analysis of various sample lysates using GTX64429 BNIP3 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BNIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64429

GTX64429 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX64429 BNIP3 antibody.
Dilution : 1:100

Anti-BNIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64429

GTX64429 IHC-P Image

IHC-P analysis of human liver damage tissue using GTX64429 BNIP3 antibody.
Dilution : 1:100

Anti-BNIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64429

GTX64429 IHC-P Image

IHC-P analysis of rat heart tissue using GTX64429 BNIP3 antibody.
Dilution : 1:100

Anti-BNIP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64429

GTX64429 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX64429 BNIP3 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399