APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BNIP3L. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909999
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL2 interacting protein 3 like , BNIP3a , NIX
Cellular Localization
Endoplasmic reticulum,Mitochondrion outer membrane,Membrane
Background
This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c. [provided by RefSeq, Feb 2015]
Database
Research Area
DATA IMAGES
![Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515](/templates/default/js/lightbox/images/loading.gif "Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515")
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GTX636515 IHC-P Image
BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.BNIP3L stained by BNIP3L antibody [HL1203] (GTX636515) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636515 IHC-P Image
BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.BNIP3L stained by BNIP3L antibody [HL1203] (GTX636515) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636515 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody [HL1203] (GTX636515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636515 IHC-P Image
BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.BNIP3L stained by BNIP3L antibody [HL1203] (GTX636515) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636515 ICC/IF Image
BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BNIP3L stained by BNIP3L antibody [HL1203] (GTX636515) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636515 ICC/IF Image
BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BNIP3L stained by BNIP3L antibody [HL1203] (GTX636515) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636515 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody [HL1203] (GTX636515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for BNIP3L antibody [HL1203] (GTX636515). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BNIP3L antibody [HL1203] (GTX636515). Be the first to share your experience with this product.
![Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515](/upload/website/prouct_img/small/GTX636515/GTX636515_44501_20221230_IHC-P_multiple_M_22122821_427.webp "Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515](/upload/website/prouct_img/small/GTX636515/GTX636515_44501_20221230_IHC-P_multiple_R_22122821_684.webp "Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in Western Blot (WB). GTX636515](/upload/website/prouct_img/small/GTX636515/GTX636515_44501_20220211_WB_R_w_23061202_137.webp "Anti-BNIP3L antibody [HL1203] used in Western Blot (WB). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515](/upload/website/prouct_img/small/GTX636515/GTX636515_44501_20220318_IHC-P_M_w_23061202_197.webp "Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636515](/upload/website/prouct_img/small/GTX636515/GTX636515_44501_20220318_ICC_IF_treatment_CoCl2_w_23061202_154.webp "Anti-BNIP3L antibody [HL1203] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636515](/upload/website/prouct_img/small/GTX636515/GTX636515_44501_20220128_ICC_IF_w_23061202_550.webp "Anti-BNIP3L antibody [HL1203] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in Western Blot (WB). GTX636515](/upload/website/prouct_img/small/GTX636515/GTX636515_44501_20211119_WB_treatment_CoCl2_w_23061202_892.webp "Anti-BNIP3L antibody [HL1203] used in Western Blot (WB). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515](/upload/website/prouct_img/normal/GTX636515/GTX636515_44501_20221230_IHC-P_multiple_M_22122821_427.webp "Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515](/upload/website/prouct_img/normal/GTX636515/GTX636515_44501_20221230_IHC-P_multiple_R_22122821_684.webp "Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in Western Blot (WB). GTX636515](/upload/website/prouct_img/normal/GTX636515/GTX636515_44501_20220211_WB_R_w_23061202_137.webp "Anti-BNIP3L antibody [HL1203] used in Western Blot (WB). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515](/upload/website/prouct_img/normal/GTX636515/GTX636515_44501_20220318_IHC-P_M_w_23061202_197.webp "Anti-BNIP3L antibody [HL1203] used in IHC (Paraffin sections) (IHC-P). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636515](/upload/website/prouct_img/normal/GTX636515/GTX636515_44501_20220318_ICC_IF_treatment_CoCl2_w_23061202_154.webp "Anti-BNIP3L antibody [HL1203] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636515](/upload/website/prouct_img/normal/GTX636515/GTX636515_44501_20220128_ICC_IF_w_23061202_550.webp "Anti-BNIP3L antibody [HL1203] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636515")
![Anti-BNIP3L antibody [HL1203] used in Western Blot (WB). GTX636515](/upload/website/prouct_img/normal/GTX636515/GTX636515_44501_20211119_WB_treatment_CoCl2_w_23061202_892.webp "Anti-BNIP3L antibody [HL1203] used in Western Blot (WB). GTX636515")