BNIP3L antibody

Cat. No. GTX111876

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Bovine
Package	100 μl ($419), 25 μl ($169)

See all BNIP3L products

View product citations for antibody GTX111876 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Zebrafish, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BNIP3L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036357

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 interacting protein 3 like , BNIP3a , NIX

Cellular Localization

Nucleus envelope , Endoplasmic reticulum , Mitochondrion , Membrane

Background

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This protein may function simultaneously with BNIP3 and may play a role in tumor suppression. [provided by RefSeq]

Database

Gene ID: 665 BNIP3L
UniProt: O60238 BNIP3L

Research Area

DATA IMAGES

GTX111876 IHC-P Image

BNIP3L antibody detects BNIP3L protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BNIP3L stained by BNIP3L antibody (GTX111876) diluted at 1:750.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111876 IHC-P Image

BNIP3L antibody detects BNIP3L protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BNIP3L stained by BNIP3L antibody (GTX111876) diluted at 1:750.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111876 IHC-P Image

BNIP3L antibody detects BNIP3L protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BNIP3L stained by BNIP3L antibody (GTX111876) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111876 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BNIP3L antibody (GTX111876) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111876 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using BNIP3L(GTX111876) antibody at 1:200 dilution.

GTX111876 IHC-P Image

BNIP3L antibody detects BNIP3L protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
BNIP3L stained by BNIP3L antibody (GTX111876) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min