Western blot at 0.5 to 1 μg/ml.
K562 cell lysatecan be used as positive control and the approximate 40kDa top band represents monomer of Bnip3L (4).
PBS with 0.02% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5mg/ml(Please refer to the vial label for the specific concentration.)
peptide (DAQHESGQSSSRGSSH)corresponding to amino acids 77 to 92 of human origin,which are identical to those of mouse Bnip3L (3).
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designatedBnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.). Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA ofBnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
GTX28399 WB Image
Western blot analysis of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide with Bnip3L antibody (GTX28399) at 1μg/ml.
GTX28399 IHC-P Image
Immunohistochemical staining of human kidney tissue using Bnip3L antibody (GTX28399) at 2μg/ml.