GeneTex
United States (US)

BNIP3L antibody

BNIP3L-antibody_WB_GTX28399-1_18121409_962.jpg
BNIP3L-antibody_IHC-P_GTX28399-1_18121409_290.jpg

Cat No. GTX28399

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

Western blot at 0.5 to 1 μg/ml.

Calculated MW

24 kDa. ( Note )

Positive Control

K562 cell lysatecan be used as positive control and the approximate 40kDa top band represents monomer of Bnip3L (4).
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

peptide (DAQHESGQSSSRGSSH)corresponding to amino acids 77 to 92 of human origin,which are identical to those of mouse Bnip3L (3).

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designatedBnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.). Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA ofBnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

Database

Research Area

DATA IMAGES
BNIP3L-antibody_WB_GTX28399-1_18121409_962.jpg

GTX28399 WB Image

Western blot analysis of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide with Bnip3L antibody (GTX28399) at 1μg/ml.

BNIP3L-antibody_IHC-P_GTX28399-1_18121409_290.jpg

GTX28399 IHC-P Image

Immunohistochemical staining of human kidney tissue using Bnip3L antibody (GTX28399) at 2μg/ml.