GeneTex

  • Country / Location Selection

United States (US)

BNP antibody [N1C3]

Anti-BNP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100538
Anti-BNP antibody [N1C3] used in Western Blot (WB). GTX100538

Cat. No. GTX100538

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all BNP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BNP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240520

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

natriuretic peptide B , BNP

Cellular Localization

Secreted

Background

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BNP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100538

GTX100538 ICC/IF Image

BNP antibody [N1C3] detects BNP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BNP protein stained by BNP antibody [N1C3] (GTX100538) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-BNP antibody [N1C3] used in Western Blot (WB). GTX100538

GTX100538 WB Image

BNP antibody detects BNP protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BNP antibody (GTX100538) diluted by 1:1000.

REFERENCE

There are currently no references for BNP antibody [N1C3] (GTX100538). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for BNP antibody [N1C3] (GTX100538). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169