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BNP antibody [N1C3]

Anti-BNP antibody [N1C3] used in Western Blot (WB). GTX100538
Anti-BNP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100538

Cat No. GTX100538

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BNP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

natriuretic peptide B , BNP

Cellular Localization

Secreted

Background

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-BNP antibody [N1C3] used in Western Blot (WB). GTX100538

GTX100538 WB Image

BNP antibody detects BNP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BNP antibody (GTX100538) diluted by 1:1000.

Anti-BNP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100538

GTX100538 ICC/IF Image

BNP antibody [N1C3] detects BNP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BNP protein stained by BNP antibody [N1C3] (GTX100538) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
There are currently no references for BNP antibody [N1C3] (GTX100538). Be the first to share your publications with this product.
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169