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BOB1 antibody [BOB1/2424]

Anti-BOB1 antibody [BOB1/2424] used in Flow cytometry (FACS). GTX17982
Anti-BOB1 antibody [BOB1/2424] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17982
Anti-BOB1 antibody [BOB1/2424] used in Western Blot (WB). GTX17982
Anti-BOB1 antibody [BOB1/2424] used in Protein Array (Protein Array). GTX17982
Anti-BOB1 antibody [BOB1/2424] used in IHC (Paraffin sections) (IHC-P). GTX17982

Cat No. GTX17982

Host

Mouse

Clonality

Monoclonal

Clone Name

BOB1/2424

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, FACS, Protein Array

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/ml
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BOB1 , OBF1 , OCAB , POU class 2 associating factor 1 , POU2AF1 , OCA-B , Bob 1 , POU class 2 homeobox associating factor 1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-BOB1 antibody [BOB1/2424] used in Flow cytometry (FACS). GTX17982

GTX17982 FACS Image

FACS analysis of paraformaldehyde-fixed Raji cells using GTX17982 BOB1 antibody [BOB1/2424].
Blue : Primary antibody
Red : Isotype control

Anti-BOB1 antibody [BOB1/2424] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17982

GTX17982 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed Raji cells using GTX17982 BOB1 antibody [BOB1/2424].
Green : Primary antibody
Red : nucleus

Anti-BOB1 antibody [BOB1/2424] used in Western Blot (WB). GTX17982

GTX17982 WB Image

WB analysis of Raji cell lysate using GTX17982 BOB1 antibody [BOB1/2424].

Anti-BOB1 antibody [BOB1/2424] used in Protein Array (Protein Array). GTX17982

GTX17982 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-BOB1 antibody [BOB1/2424] used in IHC (Paraffin sections) (IHC-P). GTX17982

GTX17982 IHC-P Image

IHC-P analysis of human spleen tissue using GTX17982 BOB1 antibody [BOB1/2424].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379