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BOLL antibody, C-term

Anti-BOLL antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89597

Cat. No. GTX89597

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all BOLL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 4-6μg/ml

Note :

IHC-P
Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia.
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89597-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_932074.1; NP_149019.1).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QPEPIKTVWSIHY, from the C Terminus of the protein sequence according to NP_932074.1; NP_149019.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

boule homolog, RNA binding protein , BOULE

Cellular Localization

Cytoplasm

Background

This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BOLL antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89597

GTX89597 IHC-P Image

IHC-P analysis of human testis using GTX89597 BOLL antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399