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BOLL antibody

Anti-BOLL antibody used in IHC (Paraffin sections) (IHC-P). GTX116790
Anti-BOLL antibody used in Western Blot (WB). GTX116790
Anti-BOLL antibody used in IHC (Paraffin sections) (IHC-P). GTX116790

Cat. No. GTX116790

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all BOLL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BOLL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624528

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

boule homolog, RNA binding protein , BOULE

Cellular Localization

Cytoplasm

Background

This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BOLL antibody used in IHC (Paraffin sections) (IHC-P). GTX116790

GTX116790 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BOLL(GTX116790) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BOLL antibody used in Western Blot (WB). GTX116790

GTX116790 WB Image

BOLL antibody detects BOLL protein by Western blot analysis.
A. 30 μg NT2D1 whole cell lysate/extract
12 % SDS-PAGE
BOLL antibody (GTX116790) dilution: 1:500

Anti-BOLL antibody used in IHC (Paraffin sections) (IHC-P). GTX116790

GTX116790 IHC-P Image

BOLL antibody detects BOLL protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
BOLL antibody (GTX116790) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

BOLL antibody Cat. No. GTX116790

Rating
( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-BOLL antibody used in IHC (Paraffin sections) (IHC-P). GTX116790
Date : Anonymous submitted on 19-Jan-2016
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : testis tissue
Blocking : 1.5% normal goat serum, 25°C, 1Hr
Permeabilization /Antigen retrieval : 10mM citrate buffer pH6.0
Primary Antibody : 1:100, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169