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BPDE-DNA antibody [5D11]

Cat. No. GTX54510

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D11

Isotype

 IgG2a

Application

 ICC/IF, IHC-P, FACS, IP, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all BPDE-DNA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent

Note :

IHC-P
5 μm sections were RNase and prot-K treated. DNA was denatured with 4N HCl and neutralized with 50mM Tris base. Section was blocked with 1.5% normal horse serum.

FACS
Washed sperm was fixed in 2% paraformaldehyde and permeabilized with 0.2% triton x-100/0.1% sodium citrate. Samples were treated with protK and RNase. To denature DNA samples were incubated with 4n HCl. After blocking with 5% normal serum samples were incubated with mAb.

ELISA
Plates were coated with 50 ng/well BPDE-DNA in 50mM Tris-buffer pH7.5 o/n at 4ºC. Plates were blocked 1% FCS. DNA samples, 4μg, were mixed with 5D11 and added to the well. Detection with GtαMs-IgG-AP for 90 mins at 37ºC.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

BPDE-I-DNA complexed with methylated BSA

Purification

Purified IgG2a

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

REFERENCE

Clone Reference

Gu A et al. Toxicol Sci 2012; 128 (2):357-64 Contributions of aryl hydrocarbon receptor genetic variants to the risk of glioma and PAH-DNA adducts
Application : IHC-P
Reactivity : Human
Perrin J et al. Fertil Steril 2011; 95 (6):2013-7 Tobacco consumption and benzo(a)pyrene-diol-epoxide-DNA adducts in spermatozoa: in smokers, swim-up procedure selects spermatozoa with decreased DNA damage
Reactivity : Human
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Package List Price ($)
$ 399