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BPGM antibody [N1C3]

Anti-BPGM antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107137
Anti-BPGM antibody [N1C3] used in Western Blot (WB). GTX107137
Anti-BPGM antibody [N1C3] used in Western Blot (WB). GTX107137

Cat No. GTX107137

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , Molt-4 , Raji , mouse spleen

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BPGM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bisphosphoglycerate Mutase,Dpgm,Bpgm

Background

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-BPGM antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107137

GTX107137 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BPGM(GTX107137) antibody at 1:100 dilution.

Anti-BPGM antibody [N1C3] used in Western Blot (WB). GTX107137

GTX107137 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
12% SDS PAGE
GTX107137 diluted at 1:1000

Anti-BPGM antibody [N1C3] used in Western Blot (WB). GTX107137

GTX107137 WB Image

Sample (50 ug of whole cell lysate)
A: mouse spleen
12% SDS PAGE
GTX107137 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169