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BPGM antibody

Anti-BPGM antibody used in IHC (Paraffin sections) (IHC-P). GTX33041
Anti-BPGM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33041
Anti-BPGM antibody used in Western Blot (WB). GTX33041

Cat. No. GTX33041

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all BPGM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human BPGM (NP_001715.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bisphosphoglycerate mutase , DPGM , ECYT8

Background

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-BPGM antibody used in IHC (Paraffin sections) (IHC-P). GTX33041

GTX33041 IHC-P Image

IHC-P analysis of human stomach tissue using GTX33041 BPGM antibody.
Dilution : 1:100

Anti-BPGM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33041

GTX33041 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33041 BPGM antibody.
Blue : DAPI
Dilution : 1:100

Anti-BPGM antibody used in Western Blot (WB). GTX33041

GTX33041 WB Image

WB analysis of various sample lysates using GTX33041 BPGM antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399