GeneTex
United States (US)

BPNT1 antibody [N1C3]

GTX104970_39771_IHC_M_2_19011506_264.jpg
GTX104970_39771_IHC_M_19011506_102.jpg
GTX104970_39771_WB_M_Liver_19011506_730.jpg
GTX104970_WB_19011506_854.jpg

Cat No. GTX104970

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLaS3 , mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BPNT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PIP antibody, BPNT1 antibody, bisphosphate 3'-nucleotidase 1 antibody, BPntase antibody, "PAP-inositol-1,4-phosphatase antibody", "3'(2'),5'-bisphosphate nucleotidase 1 antibody", "3'(2'), 5'-bisphosphate nucleotidase 1 antibody"

Background

BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq]

Database

DATA IMAGES
GTX104970_39771_IHC_M_2_19011506_264.jpg

GTX104970 IHC-P Image

BPNT1 antibody [N1C3] detects BPNT1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BPNT1 antibody [N1C3] (GTX104970) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104970_39771_IHC_M_19011506_102.jpg

GTX104970 IHC-P Image

BPNT1 antibody [N1C3] detects BPNT1 protein at cytosol on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
BPNT1 antibody [N1C3] (GTX104970) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104970_39771_WB_M_Liver_19011506_730.jpg

GTX104970 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
12% SDS PAGE
GTX104970 diluted at 1:1000

GTX104970_WB_19011506_854.jpg

GTX104970 WB Image

Sample (30μg whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX104970 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169