APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLaS3 , mouse liver
Predict Reactivity
Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.7mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human BPNT1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
PIP antibody, BPNT1 antibody, bisphosphate 3'-nucleotidase 1 antibody, BPntase antibody, "PAP-inositol-1,4-phosphatase antibody", "3'(2'),5'-bisphosphate nucleotidase 1 antibody", "3'(2'), 5'-bisphosphate nucleotidase 1 antibody"
Background
BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq]
Database
DATA IMAGES
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GTX104970 IHC-P Image
BPNT1 antibody [N1C3] detects BPNT1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BPNT1 antibody [N1C3] (GTX104970) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104970 IHC-P Image
BPNT1 antibody [N1C3] detects BPNT1 protein at cytosol on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
BPNT1 antibody [N1C3] (GTX104970) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104970 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse liver
12% SDS PAGE
GTX104970 diluted at 1:1000
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GTX104970 WB Image
Sample (30μg whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX104970 diluted at 1:500
|
