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BPNT1 antibody [N1C3]

Anti-BPNT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104970
Anti-BPNT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104970
Anti-BPNT1 antibody [N1C3] used in Western Blot (WB). GTX104970
Anti-BPNT1 antibody [N1C3] used in Western Blot (WB). GTX104970

Cat No. GTX104970

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all BPNT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HeLaS3 , mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BPNT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

3'(2'), 5'-bisphosphate nucleotidase 1 , HEL20 , PIP

Background

BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BPNT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104970

GTX104970 IHC-P Image

BPNT1 antibody [N1C3] detects BPNT1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BPNT1 antibody [N1C3] (GTX104970) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BPNT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104970

GTX104970 IHC-P Image

BPNT1 antibody [N1C3] detects BPNT1 protein at cytosol on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
BPNT1 antibody [N1C3] (GTX104970) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BPNT1 antibody [N1C3] used in Western Blot (WB). GTX104970

GTX104970 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
12% SDS PAGE
GTX104970 diluted at 1:1000

Anti-BPNT1 antibody [N1C3] used in Western Blot (WB). GTX104970

GTX104970 WB Image

Sample (30μg whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX104970 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169