APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 3-5μg/ml |
|
Note :
IHC-P
Human Heart shows staining of myocardial fibres in transverse section.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89774-PEP).
Product Note
This antibody is expected to recognise all reported isoforms (NP_115937.1; NP_742024.1; NP_742025.1).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence RALEDLLVSIGLDC, from the C Terminus of the protein sequence according to NP_115937.1; NP_742024.1; NP_742025.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Ankyrin Repeat And Btb Domain Containing 1 , Bpoz , Btb3 , Btbd21 , Ef1Abp , Pp2259 , Abtb1
Background
This gene encodes a protein with an ankyrin repeat region and two BTB/POZ domains, which are thought to be involved in protein-protein interactions. Expression of this gene is activated by the phosphatase and tensin homolog, a tumor suppressor. Alternate splicing results in three transcript variants. [provided by RefSeq, Mar 2010]
Research Area
DATA IMAGES
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GTX89774 IHC-P Image
IHC-P analysis of human heart using GTX89774 BPOZ antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
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GTX89774 IHC-P Image
IHC-P analysis of paraffin embedded human cerebellum using GTX89774 BPOZ antibody, C-term.
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89774 WB Image
WB analysis of human heart lysate using GTX89774 BPOZ antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for BPOZ antibody, C-term (GTX89774). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BPOZ antibody, C-term (GTX89774). Be the first to share your experience with this product.
