Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX115399 diluted at 1:2000

BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of BRAF35 by 3 μg of BRAF35 antibody (GTX115399) 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody (GTX115399) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using HMG20B(GTX115399) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min