GeneTex
United States (US)

BRAF35 antibody [N2C3]

GTX115399_40302_WB_18121410_211.jpg
GTX115399_40302_IP_18121410_707.jpg
GTX115399_40302_IHC_18121410_563.jpg

Cat No. GTX115399

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BRAF35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

High Mobility Group 20B,Braf25,Braf35,Hmgx2,Hmgxb2,Pp7706,Smarce1R,Soxl,Pp8857,Hmg20B

Background

Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.

Database

Research Area

DATA IMAGES
GTX115399_40302_WB_18121410_211.jpg

GTX115399 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX115399 diluted at 1:2000

GTX115399_40302_IP_18121410_707.jpg

GTX115399 IP Image

BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of BRAF35 by 3 μg of BRAF35 antibody (GTX115399) 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody (GTX115399) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX115399_40302_IHC_18121410_563.jpg

GTX115399 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using HMG20B(GTX115399) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169