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BRAF35 antibody

Anti-BRAF35 antibody used in Immunoprecipitation (IP). GTX104102
Anti-BRAF35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104102
Anti-BRAF35 antibody used in Western Blot (WB). GTX104102

Cat No. GTX104102

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BRAF35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

high mobility group 20B , BRAF25 , BRAF35 , HMGX2 , HMGXB2 , PP7706 , SMARCE1r , SOXL , pp8857

Cellular Localization

Nucleus

Background

Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.

Database

Research Area

DATA IMAGES
Anti-BRAF35 antibody used in Immunoprecipitation (IP). GTX104102

GTX104102 IP Image

BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of BRAF35 protein by 2 μg of BRAF35 antibody (GTX104102) 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody (GTX104102) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-BRAF35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104102

GTX104102 ICC/IF Image

BRAF35 antibody detects BRAF35 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRAF35 protein stained by BRAF35 antibody (GTX104102) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-BRAF35 antibody used in Western Blot (WB). GTX104102

GTX104102 WB Image

Sample(30 ug whole cell lysate)
A: 293T
12% SDS PAGE
GTX104102 diluted at 1:500

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169