BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of BRAF35 protein by 2 μg of BRAF35 antibody (GTX104102) 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody (GTX104102) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample(30 ug whole cell lysate)
A: 293T
12% SDS PAGE
GTX104102 diluted at 1:500

BRAF35 antibody detects BRAF35 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRAF35 protein stained by BRAF35 antibody (GTX104102) diluted at 1:500.
Blue: Hoechst 33342 staining.