GeneTex
United States (US)

BRAF35 antibody

Cat No. GTX30418

Host Rabbit
Clonality Polyclonal
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

Western blot – Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Calculated MW

36 kDa. ( Note )

Specificity/Sensitivity

Reacts with residues 31-50 [KQERGEGPRA GEKGSHEEEP] of the human structural DNA-binding protein BRAF35. Amino acid identity to mouse = 16/20 (80%).
PROPERTIES

Form

Liquid

Buffer

PBS containing 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Residues 31-50 [KQERGEGPRA GEKGSHEEEP] of the human structural DNA-binding protein BRAF35.

Purification

IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Mutations of the human BRCA2 gene confer susceptibility to breast cancer. A structural DNA binding component has been identified and is designated as BRCA2-Associated Factor 35 (BRAF35). There is a close association of the BRAF35/BRCA2 complex with condensed chromatin coincident with histone H3 phosphorylation. Experimental evidence suggests a role for the BRCA2/BRAF35 complex in the modulation of cell cycle progression.

Database

Research Area

Package List Price ($)
$ 289