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BRCA1 antibody [6B4] - ChIP grade

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunoprecipitation (IP). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in ChIP assay (ChIP assay). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunohistochemistry (IHC). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

Cat No. GTX70115

Host

Mouse

Clonality

Monoclonal

Clone Name

6B4

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, ChIP assay, IHC

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

220 kDa. ( Note )

Positive Control

293T , MCF7 , MEF

Specificity/Sensitivity

This antibody recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Brca1, Dna Repair Associated , Brcai , Brcc1 , Brovca1 , Fancs , Iris , Pnca4 , Ppp1R53 , Pscp , Rnf53 , Brca1

Cellular Localization

Nuclear

Background

BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.

Database

Research Area

DATA IMAGES

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunoprecipitation (IP). GTX70115

GTX70115 IP Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BRCA1 antibody [6B4] - ChIP grade used in ChIP assay (ChIP assay). GTX70115

GTX70115 ChIP assay Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 microgram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

BRCA1 antibody 6B4 (GTX70115) was used for western blot assay at 1:1000 antibody dilution.
western blot assay was performed using MCF7 (human) and MEF(mouse embryonic fibroblast) cell lysate separated in a 5-15% gradient gel.
Observed molecular weight of BRCA1: 220 KD.
Predicted molecular weight of BRCA1: 220 KD.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70115

GTX70115 ICC/IF Image

BRCA1 antibody 6B4 (GTX70115) was used for immunofluorescent staining of BRCA1 nuclear foci induced by ionizing radiation.
IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining.
DAPI was used to counterstain nucleus.
6B4 was used at 1:400 dilution.
Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4).

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunohistochemistry (IHC). GTX70115

GTX70115 IHC Image

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

BRCA1 antibody [6B4] (GTX70115) was used at 1:1000 dilution for western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody [13B3] (GTX70228) was used as a loading control.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

BRCA1 antibody detects BRCA1 protein by western blot analysis. Whole cell extracts (30 and 50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody (GTX70115) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 329