APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human BRCA1 (AA: 229-335) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53
Cellular Localization
Nucleus,Cytoplasm
Background
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]
Database
Research Area
DATA IMAGES
![Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779](/templates/default/js/lightbox/images/loading.gif "Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779")
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GTX60779 IHC-P Image
IHC-P analysis of human esophagus tissue using GTX60779 BRCA1 antibody [6C6D2].
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GTX60779 IHC-P Image
IHC-P analysis of esophagus cancer tissue using GTX60779 BRCA1 antibody [6C6D2].
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GTX60779 WB Image
WB analysis of HEK293 (1) and BRCA1 (AA: 229-335)-hIgGFc transfected HEK293 (2) cell lysate using GTX60779 BRCA1 antibody [6C6D2].
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GTX60779 ELISA Image
ELISA analysis of antigen using GTX60779 BRCA1 antibody [6C6D2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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REFERENCE
There are currently no references for BRCA1 antibody [6C6D2] (GTX60779). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BRCA1 antibody [6C6D2] (GTX60779). Be the first to share your experience with this product.
![Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779](/upload/website/prouct_img/small/GTX60779/GTX60779_20170912_IHC-P_1_w_23061123_613.webp "Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779")
![Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779](/upload/website/prouct_img/small/GTX60779/GTX60779_20170912_IHC-P_w_23061123_199.webp "Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779")
![Anti-BRCA1 antibody [6C6D2] used in Western Blot (WB). GTX60779](/upload/website/prouct_img/small/GTX60779/GTX60779_20170912_WB_w_23061123_193.webp "Anti-BRCA1 antibody [6C6D2] used in Western Blot (WB). GTX60779")
![Anti-BRCA1 antibody [6C6D2] used in ELISA (ELISA). GTX60779](/upload/website/prouct_img/small/GTX60779/GTX60779_20170912_ELISA_w_23061123_439.webp "Anti-BRCA1 antibody [6C6D2] used in ELISA (ELISA). GTX60779")
![Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779](/upload/website/prouct_img/normal/GTX60779/GTX60779_20170912_IHC-P_1_w_23061123_613.webp "Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779")
![Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779](/upload/website/prouct_img/normal/GTX60779/GTX60779_20170912_IHC-P_w_23061123_199.webp "Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779")
![Anti-BRCA1 antibody [6C6D2] used in Western Blot (WB). GTX60779](/upload/website/prouct_img/normal/GTX60779/GTX60779_20170912_WB_w_23061123_193.webp "Anti-BRCA1 antibody [6C6D2] used in Western Blot (WB). GTX60779")
![Anti-BRCA1 antibody [6C6D2] used in ELISA (ELISA). GTX60779](/upload/website/prouct_img/normal/GTX60779/GTX60779_20170912_ELISA_w_23061123_439.webp "Anti-BRCA1 antibody [6C6D2] used in ELISA (ELISA). GTX60779")