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BRCA1 antibody [6C6D2]

Anti-BRCA1 antibody [6C6D2] used in ELISA (ELISA). GTX60779
Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779
Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779
Anti-BRCA1 antibody [6C6D2] used in Western Blot (WB). GTX60779

Cat No. GTX60779

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6C6D2

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($379)
See all BRCA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human BRCA1 (AA: 229-335) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-BRCA1 antibody [6C6D2] used in ELISA (ELISA). GTX60779

GTX60779 ELISA Image

ELISA analysis of antigen using GTX60779 BRCA1 antibody [6C6D2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779

GTX60779 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX60779 BRCA1 antibody [6C6D2].

Anti-BRCA1 antibody [6C6D2] used in IHC (Paraffin sections) (IHC-P). GTX60779

GTX60779 IHC-P Image

IHC-P analysis of esophagus cancer tissue using GTX60779 BRCA1 antibody [6C6D2].

Anti-BRCA1 antibody [6C6D2] used in Western Blot (WB). GTX60779

GTX60779 WB Image

WB analysis of HEK293 (1) and BRCA1 (AA: 229-335)-hIgGFc transfected HEK293 (2) cell lysate using GTX60779 BRCA1 antibody [6C6D2].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379