For IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Optimal dilutions/concentrations should be determined by the researcher.
This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.
Phosphate-buffered saline, pH 7.2, containing no preservatives
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
Protein fragment expressed in E. coli corresponding to amino acids 341-748.
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.