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BRCA1 antibody [8F7]

Anti-BRCA1 antibody [8F7] used in Western Blot (WB). GTX70113
Anti-BRCA1 antibody [8F7] used in IHC (Paraffin sections) (IHC-P). GTX70113

Cat No. GTX70113

Host

Mouse

Clonality

Monoclonal

Clone Name

8F7

Isotype

IgG1

Application

WB, IHC-P, IP, IHC

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

For IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

220 kDa. ( Note )

Positive Control

293T

Specificity/Sensitivity

This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 341-748.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Brca1, Dna Repair Associated , Brcai , Brcc1 , Brovca1 , Fancs , Iris , Pnca4 , Ppp1R53 , Pscp , Rnf53 , Brca1

Cellular Localization

Nuclear/Cytoplasmic

Background

BRCA1-8F7 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.

Database

Research Area

DATA IMAGES

Anti-BRCA1 antibody [8F7] used in Western Blot (WB). GTX70113

GTX70113 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] (GTX70113) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [8F7] used in IHC (Paraffin sections) (IHC-P). GTX70113

GTX70113 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Breast ca, using BRCA1(GTX70113) antibody at 1:200 dilution.

REFERENCE

REVIEW

BRCA1 antibody [8F7] Cat No. GTX70113

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-Nov-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was pear reviewed and published in the journal Aging Cell in 2015.PMID: 25645366
Date : Anonymous submitted on 19-Nov-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was pear reviewed and published in the journal Mol Cancer in 2014.PMID: 25362534
Application Reference
SDS
PBS.pdf