GeneTex
United States (US)

BRCA1 antibody [8F7]

GTX70113_40004_20161117_WB_shRNA_watermark_18121411_279.jpg
BRCA1-antibody-8F7-GTX70113-IHC-1_18121409_833.jpg

Cat No. GTX70113

Host Mouse
Clonality Monoclonal
Clone Name 8F7
Isotype IgG1
Application WB, IHC-P, IP, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

For IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

220 kDa. ( Note )

Positive Control

293T

Specificity/Sensitivity

This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 341-748.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Brca1, Dna Repair Associated,Brcai,Brcc1,Brovca1,Fancs,Iris,Pnca4,Ppp1R53,Pscp,Rnf53,Brca1

Cellular Localization

Nuclear/Cytoplasmic

Background

BRCA1-8F7 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.

Database

Research Area

DATA IMAGES
GTX70113_40004_20161117_WB_shRNA_watermark_18121411_279.jpg

GTX70113 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] (GTX70113) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

BRCA1-antibody-8F7-GTX70113-IHC-1_18121409_833.jpg

GTX70113 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Breast ca, using BRCA1(GTX70113) antibody at 1:200 dilution.

REFERENCE
Application Reference