GeneTex
United States (US)

BRCA1 antibody [BRCA1/1398]

Anti-BRCA1 antibody [BRCA1/1398] used in IHC (Paraffin sections) (IHC-P). GTX34445

Cat No. GTX34445

Host Mouse
Clonality Monoclonal
Clone Name BRCA1/1398
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human
See all BRCA1 products;
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 5-10ug/ml for 60 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

Specificity/Sensitivity

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human BRCA1 protein fragment (aa445-620) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Brca1, Dna Repair Associated,Brcai,Brcc1,Brovca1,Fancs,Iris,Pnca4,Ppp1R53,Pscp,Rnf53,Brca1

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES
Anti-BRCA1 antibody [BRCA1/1398] used in IHC (Paraffin sections) (IHC-P). GTX34445

GTX34445 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with BRCA1 Monoclonal Antibody (BRCA1/1398).
Package List Price ($)
$ 329