GeneTex
United States (US)

BRCA1 antibody [C3], C-term

Orthogonal
GTX100720_39988_IFA_19011506_375.jpg
GTX100720_39988_20180504_WB_Fraction_19011506_785.jpg

Cat No. GTX100720

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

Positive Control

A549 , A549 nuclear extract

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BRCA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Brca1, Dna Repair Associated,Brcai,Brcc1,Brovca1,Fancs,Iris,Pnca4,Ppp1R53,Pscp,Rnf53,Brca1

Cellular Localization

Nucleus , Isoform 3: Cytoplasm , Isoform 5: Cytoplasm

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100720_39988_IFA_19011506_375.jpg

GTX100720 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using BRCA1(GTX100720) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX100720_39988_20180504_WB_Fraction_19011506_785.jpg

GTX100720 WB Image

Orthogonal

A549 whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [C3], C-term (GTX100720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf