GeneTex

  • Country / Location Selection

United States (US)

BRCA1 antibody [GLK-2]

Anti-BRCA1 antibody [GLK-2] used in IHC (Paraffin sections) (IHC-P). GTX17251

Cat. No. GTX17251

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GLK-2

Isotype

 IgM

Application

 IHC-P

Reactivity

 Human
Package
250 μl ($399)
See all BRCA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:15-1:50

Note :

IHC-P
Recommendations for antigen retrieval : Citrate Buffer pH 6.0 at room temperature for 30 mins.
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

Product Note

This antibody stains 94% of ovarian carcinomas with a mutation in exon 11. However, staining was absent in 100% of tumors with mutations other than exon 11.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to amino acids 1839-1863 of the C-terminus of BRCA1.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-BRCA1 antibody [GLK-2] used in IHC (Paraffin sections) (IHC-P). GTX17251

GTX17251 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX17251 BRCA1 antibody [GLK-2].

REFERENCE

There are currently no references for BRCA1 antibody [GLK-2] (GTX17251). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for BRCA1 antibody [GLK-2] (GTX17251). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399