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BRCA1 antibody

Anti-BRCA1 antibody used in Immunohistochemistry (IHC). GTX70134
Anti-BRCA1 antibody used in Western Blot (WB). GTX70134

Cat. No. GTX70134

Reference 1

Host

 Mouse

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, IHC

Reactivity

 Human
Package
50 μl ($399)
See all BRCA1 products

APPLICATION

Application Note

For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Predicted molecular weight: 208 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

208 kDa. ( Note )

Positive Control

HBL100

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide from the human BRCA1 protein corresponding to carboxyl terminal amino acids 1848-1863 (QELDTYLIPQIPHSHY).

Purification

Unpurified
From polyclonal serum.

Conjugation

Unconjugated

RRID

AB_368626

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-BRCA1 antibody used in Immunohistochemistry (IHC). GTX70134

GTX70134 IHC Image

Anti-BRCA1 antibody used in Western Blot (WB). GTX70134

GTX70134 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody (GTX70134) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Fustier P et al. Br J Cancer 2003; 89 (1) : 168-72 Resveratrol increases BRCA1 and BRCA2 mRNA expression in breast tumour cell lines.
Application : IP
Reactivity : Human
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Package List Price ($)
$ 399