APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:50-1:200 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH 7.3, 50% glycerol
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic phosphorylated peptide around S1423 of human BRCA1 (NP_009225.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53
Cellular Localization
Nucleus , Cytoplasm
Background
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]
Database
Research Area
DATA IMAGES
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GTX03784 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX03784 BRCA1 (phospho Ser1423) antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:100
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GTX03784 IHC-P Image
IHC-P analysis of human breast cancer tissue using GTX03784 BRCA1 (phospho Ser1423) antibody.
Dilution : 1:100
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GTX03784 WB Image
WB analysis of HT-29 cell treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight lysates using GTX03784 BRCA1 (phospho Ser1423) antibody.
Dilution : 1:2000
Loading : 25 μg per lane
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GTX03784 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX03784 BRCA1 (phospho Ser1423) antibody.
Dilution : 1:100
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GTX03784 IHC-P Image
IHC-P analysis of rat testis tissue using GTX03784 BRCA1 (phospho Ser1423) antibody.
Dilution : 1:100
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REFERENCE
There are currently no references for BRCA1 (phospho Ser1423) antibody (GTX03784). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BRCA1 (phospho Ser1423) antibody (GTX03784). Be the first to share your experience with this product.
