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BRCA1 (phospho Ser1423) antibody

Anti-BRCA1 (phospho Ser1423) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03784
Anti-BRCA1 (phospho Ser1423) antibody used in IHC (Paraffin sections) (IHC-P). GTX03784
Anti-BRCA1 (phospho Ser1423) antibody used in Western Blot (WB). GTX03784
Anti-BRCA1 (phospho Ser1423) antibody used in IHC (Paraffin sections) (IHC-P). GTX03784
Anti-BRCA1 (phospho Ser1423) antibody used in IHC (Paraffin sections) (IHC-P). GTX03784

Cat No. GTX03784

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic phosphorylated peptide around S1423 of human BRCA1 (NP_009225.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-BRCA1 (phospho Ser1423) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03784

GTX03784 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX03784 BRCA1 (phospho Ser1423) antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-BRCA1 (phospho Ser1423) antibody used in IHC (Paraffin sections) (IHC-P). GTX03784

GTX03784 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX03784 BRCA1 (phospho Ser1423) antibody.
Dilution : 1:100

Anti-BRCA1 (phospho Ser1423) antibody used in Western Blot (WB). GTX03784

GTX03784 WB Image

WB analysis of HT-29 cell treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight lysates using GTX03784 BRCA1 (phospho Ser1423) antibody.
Dilution : 1:2000
Loading : 25 μg per lane

Anti-BRCA1 (phospho Ser1423) antibody used in IHC (Paraffin sections) (IHC-P). GTX03784

GTX03784 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX03784 BRCA1 (phospho Ser1423) antibody.
Dilution : 1:100

Anti-BRCA1 (phospho Ser1423) antibody used in IHC (Paraffin sections) (IHC-P). GTX03784

GTX03784 IHC-P Image

IHC-P analysis of rat testis tissue using GTX03784 BRCA1 (phospho Ser1423) antibody.
Dilution : 1:100

REFERENCE

There are currently no references for BRCA1 (phospho Ser1423) antibody (GTX03784). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for BRCA1 (phospho Ser1423) antibody (GTX03784). Be the first to share your experience with this product.
Package List Price ($)
$ 379