BRCA2 antibody [5F6]

Cat No. GTX70123

Host	Mouse
Clonality	Monoclonal
Clone Name	5F6
Isotype	IgG1
Application	WB, IHC-P, IHC-Fr
Reactivity	Human
Package	100 μl ($329)

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APPLICATION

Application Note

Do not boil samples for longer than two minutes. For WB: Use at a concentration of 1-5 μg/ml. Predicted molecular weight: 380 kDa. Optimal dilutions/concentrations should be determined by the reseracher.

Calculated MW

384 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 2336-2478 of human BRCA2 expressed in E. coli. BRCA2-P400.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368624

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BRCA2 DNA repair associated , BRCC2 , BROVCA2 , FACD , FAD , FAD1 , FANCD , FANCD1 , GLM3 , PNCA2 , XRCC11

Cellular Localization

Nucleus

Background

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]

Database

Gene ID: 675 BRCA2
UniProt: P51587 BRCA2

Research Area

DATA IMAGES

GTX70123 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX70123 diluted at 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.