GeneTex
United States (US)

BRCA2 antibody [5F6]

BRCA2-antibody-5F6-GTX70123-WB-1_18122101_163.jpg

Cat No. GTX70123

Host Mouse
Clonality Monoclonal
Clone Name 5F6
Isotype IgG1
Application WB, IHC-P, IHC-Fr, IHC
Reactivity Human
APPLICATION

Application Note

Do not boil samples for longer than two minutes. For WB: Use at a concentration of 1-5 μg/ml. Predicted molecular weight: 380 kDa. Optimal dilutions/concentrations should be determined by the reseracher.

Calculated MW

390 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, no preservative added.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 2336-2478 of human BRCA2 expressed in E. coli. BRCA2-P400.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nuclear

Background

BRCA2-5F6 recognizes the BRCA2 gene product, a 390kDa nuclear protein. The BRCA2 gene was originally isolated based on its importance in the predisposition of women to early-onset breast and ovarian cancers, suggesting mutations affect its tumor suppressor-like activity. Targeted disruption of the BRCA2 gene in mice and binding of Rad51 to its carboxyl terminus have indicated an involvement in DNA recombination and double strand break repair.

Database

Research Area

DATA IMAGES
BRCA2-antibody-5F6-GTX70123-WB-1_18122101_163.jpg

GTX70123 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX70123 diluted at 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE