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BRCA2 (phospho Ser2095) antibody

Orthogonal
Anti-BRCA2 (phospho Ser2095) antibody used in Western Blot (WB). GTX03783
Anti-BRCA2 (phospho Ser2095) antibody used in IHC (Paraffin sections) (IHC-P). GTX03783
Anti-BRCA2 (phospho Ser2095) antibody used in IHC (Paraffin sections) (IHC-P). GTX03783
Anti-BRCA2 (phospho Ser2095) antibody used in ELISA (ELISA). GTX03783
Anti-BRCA2 (phospho Ser2095) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03783

Cat. No. GTX03783

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($409)
See all BRCA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

384 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human BRCA2(Accession P51587), corresponding to amino acid residues E2081-P2107.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA2 DNA repair associated , BRCC2 , BROVCA2 , FACD , FAD , FAD1 , FANCD , FANCD1 , GLM3 , PNCA2 , XRCC11

Cellular Localization

Nucleus

Background

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-BRCA2 (phospho Ser2095) antibody used in Western Blot (WB). GTX03783

GTX03783 WB Image

WB analysis of various lysates using GTX03783 BRCA2 (phospho Ser2095) antibody.
Lane 1: Rat liver, blocked with antigen-specific peptides.
Lane 2 : Rat liver.
Lane 3 : Mouse brain
Lane 4 : Hela cells (heat-shock treatment).
Lane 5 : HepG2 (heat-shock treatment)

Anti-BRCA2 (phospho Ser2095) antibody used in IHC (Paraffin sections) (IHC-P). GTX03783

GTX03783 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX03783 BRCA2 (phospho Ser2095) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-BRCA2 (phospho Ser2095) antibody used in IHC (Paraffin sections) (IHC-P). GTX03783

GTX03783 IHC-P Image

IHC-P analysis of human spleen tissue using GTX03783 BRCA2 (phospho Ser2095) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-BRCA2 (phospho Ser2095) antibody used in ELISA (ELISA). GTX03783

GTX03783 ELISA Image

Orthogonal

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX03783 BRCA2 (phospho Ser2095) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-BRCA2 (phospho Ser2095) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03783

GTX03783 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells (LPS-treated, 4 hr) using GTX03783 BRCA2 (phospho Ser2095) antibody.
Permeabilize : 0.1% Triton X-100
Dilution : 1:200
Red : Primary antibody
Green : beta-tubulin
Blue : DAPI

REFERENCE

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REVIEW

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Package List Price ($)
$ 409