GeneTex

  • Country / Location Selection

United States (US)

BRCC36 antibody [AT3B1]

Anti-BRCC36 antibody [AT3B1] used in Western Blot (WB). GTX50015

Cat. No. GTX50015

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT3B1

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all BRCC36 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1000 ~ 2000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant BRCC36 (1-316aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA1/BRCA2-containing complex subunit 3 , BRCC36 , C6.1A , CXorf53

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-BRCC36 antibody [AT3B1] used in Western Blot (WB). GTX50015

GTX50015 WB Image

Cell lysates of HeLa(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

There are currently no references for BRCC36 antibody [AT3B1] (GTX50015). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for BRCC36 antibody [AT3B1] (GTX50015). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399