GeneTex
United States (US)

BRCC36 antibody

Anti-BRCC36 antibody used in IHC (Paraffin sections) (IHC-P). GTX31765
Anti-BRCC36 antibody used in Western Blot (WB). GTX31765
Anti-BRCC36 antibody used in IHC (Paraffin sections) (IHC-P). GTX31765

Cat No. GTX31765

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , MCF7 Cell Lysate
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BRCC36 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36.The immunogen is located within amino acids 20 - 70 of BRCC36.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Brca1/Brca2-Containing Complex Subunit 3,Brcc36,C6.1A,Cxorf53,Brcc3

Background

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES
Anti-BRCC36 antibody used in IHC (Paraffin sections) (IHC-P). GTX31765

GTX31765 IHC-P Image

IHC-P analysis of human brain tissue using GTX31765 BRCC36 antibody.
Working concentration : 20 μg/ml

Anti-BRCC36 antibody used in Western Blot (WB). GTX31765

GTX31765 WB Image

WB analysis of MCF7 cell lysate using GTX31765 BRCC36 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-BRCC36 antibody used in IHC (Paraffin sections) (IHC-P). GTX31765

GTX31765 IHC-P Image

IHC-P analysis of human brain tissue using GTX31765 BRCC36 antibody.
Working concentration : 2.5 μg/ml

REFERENCE
Package List Price ($)
$ 329