GeneTex
United States (US)

BRCC45 antibody

KO/KD
GTX105364_39911_20170629_WB_KO_watermark_19011506_280.jpg
GTX105364_39911_IP_19011506_925.jpg
GTX105364_39911_WB_19011506_875.jpg
GTX105364_39911_20170818_WB_R_testis_19011506_123.jpg
GTX105364_39911_WB_M_19011506_948.jpg
GTX105364_IFA_19011506_197.jpg

Cat No. GTX105364

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HeLa , 293T , A431 , C8D30 , rat testis

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BRCC45. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BRCC4 antibody, BRCC45 antibody, BRE antibody, BRCA1/BRCA2-containing complex subunit 45 antibody, BRCA1/BRCA2-containing complex, subunit 4 antibody, BRCA1-A complex subunit BRE antibody, brain and reproductive organ-expressed protein antibody, brain and reproductive organ-expressed (TNFRSF1A modulator) antibody

Cellular Localization

Cytoplasm , Nucleus

Background

Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between MERIT40/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.

Database

Research Area

DATA IMAGES
GTX105364_39911_20170629_WB_KO_watermark_19011506_280.jpg

GTX105364 WB Image

KO/KD

Wild-type (WT) and BRCC45 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody (GTX105364) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX105364_39911_IP_19011506_925.jpg

GTX105364 IP Image

BRE antibody immunoprecipitates BRE protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. Control with 2.5 μg of preimmune rabbit IgG B. Immunoprecipitation of BRE protein by 2.5 μg of BRE antibody (GTX105364) 10% SDS-PAGE The immunoprecipitated BRE protein was detected by BRE antibody (GTX105364) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105364_39911_WB_19011506_875.jpg

GTX105364 WB Image

BRCC45 antibody detects BRE protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
10 % SDS-PAGE
BRCC45 antibody (GTX105364) dilution: 1:1000

GTX105364_39911_20170818_WB_R_testis_19011506_123.jpg

GTX105364 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody (GTX105364) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105364_39911_WB_M_19011506_948.jpg

GTX105364 WB Image

BRCC45 antibody detects BRE protein by Western blot analysis.
A. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
BRCC45 antibody (GTX105364) dilution: 1:1000

GTX105364_IFA_19011506_197.jpg

GTX105364 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE(GTX105364) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169