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BRD3 antibody

Anti-BRD3 antibody used in Western Blot (WB). GTX115058
Anti-BRD3 antibody used in Western Blot (WB). GTX115058
Anti-BRD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115058
Anti-BRD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115058

Cat No. GTX115058

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

HeLa nucleus , mouse Liver

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BRD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724114

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

bromodomain containing 3 , ORFX , RING3L

Cellular Localization

Nucleus

Background

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BRD3 antibody used in Western Blot (WB). GTX115058

GTX115058 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD3 antibody (GTX115058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD3 antibody used in Western Blot (WB). GTX115058

GTX115058 WB Image

Sample (20 μg of whole cell lysate)
A: mouse Liver
7.5% SDS PAGE
GTX115058 diluted at 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115058

GTX115058 ICC/IF Image

BRD3 antibody detects BRD3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRD3 stained by BRD3 antibody (GTX115058) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-BRD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115058

GTX115058 IHC-P Image

BRD3 antibody detects BRD3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
BRD3 stained by BRD3 antibody (GTX115058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf