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BRD3 antibody

Orthogonal
Anti-BRD3 antibody used in Western Blot (WB). GTX115058
Anti-BRD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115058
Anti-BRD3 antibody used in Western Blot (WB). GTX115058
Anti-BRD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115058

Cat. No. GTX115058

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all BRD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Mouse liver

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BRD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724114

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bromodomain containing 3 , ORFX , RING3L

Cellular Localization

Nucleus

Background

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BRD3 antibody used in Western Blot (WB). GTX115058

GTX115058 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD3 antibody (GTX115058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115058

GTX115058 ICC/IF Image

BRD3 antibody detects BRD3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRD3 stained by BRD3 antibody (GTX115058) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-BRD3 antibody used in Western Blot (WB). GTX115058

GTX115058 WB Image

Sample (20 μg of whole cell lysate)
A: mouse Liver
7.5% SDS PAGE
GTX115058 diluted at 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115058

GTX115058 IHC-P Image

BRD3 antibody detects BRD3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
BRD3 stained by BRD3 antibody (GTX115058) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Wang SA et al. Sci Rep 2020; 10 (1) : 20870 USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169