BRD4 antibody [HL2729] detects BRD4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BRD4 antibody [HL2729] detects BRD4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.