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BRD4 antibody [HL2729]

Recombinant antibody Orthogonal
Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557
Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557
Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557
Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557
Anti-BRD4 antibody [HL2729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639557
Anti-BRD4 antibody [HL2729] used in IHC (Paraffin sections) (IHC-P). GTX639557

Cat. No. GTX639557

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2729

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BRD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human BRD4.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bromodomain containing 4 , CAP , HUNK1 , HUNKI , MCAP

Cellular Localization

Nucleus

Background

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557

GTX639557 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557

GTX639557 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557

GTX639557 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD4 antibody [HL2729] used in Western Blot (WB). GTX639557

GTX639557 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD4 antibody [HL2729] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639557

GTX639557 ICC/IF Image

BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-BRD4 antibody [HL2729] used in IHC (Paraffin sections) (IHC-P). GTX639557

GTX639557 IHC-P Image

BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations