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BRD4 antibody

Orthogonal
Anti-BRD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130586
Anti-BRD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX130586
Anti-BRD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX130586
Anti-BRD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX130586
Anti-BRD4 antibody used in Western Blot (WB). GTX130586
Anti-BRD4 antibody used in Immunoprecipitation (IP). GTX130586
Anti-BRD4 antibody used in Western Blot (WB). GTX130586
Anti-BRD4 antibody used in Western Blot (WB). GTX130586

Cat. No. GTX130586

Reference 4
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BRD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BRD4 . The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886303

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bromodomain containing 4 , CAP , HUNK1 , HUNKI , MCAP

Cellular Localization

Nucleus

Background

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BRD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130586

GTX130586 ICC/IF Image

BRD4 antibody detects BRD4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRD4 stained by BRD4 antibody (GTX130586) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-BRD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX130586

GTX130586 IHC-P Image

BRD4 antibody detects BRD4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BRD4 stained by BRD4 antibody (GTX130586) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BRD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX130586

GTX130586 IHC-P Image

BRD4 antibody detects BRD4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
BRD4 stained by BRD4 antibody (GTX130586) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BRD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX130586

GTX130586 IHC-P Image

BRD4 antibody detects BRD4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BRD4 stained by BRD4 antibody (GTX130586) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BRD4 antibody used in Western Blot (WB). GTX130586

GTX130586 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody (GTX130586) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BRD4 antibody used in Immunoprecipitation (IP). GTX130586

GTX130586 IP Image

Immunoprecipitation of BRD4 protein from 293T whole cell extract using 5 μg of BRD4 antibody (GTX130586).
Western blot analysis was performed using BRD4 antibody (GTX130586).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-BRD4 antibody used in Western Blot (WB). GTX130586

GTX130586 WB Image

Orthogonal

293T whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody (GTX130586) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD4 antibody used in Western Blot (WB). GTX130586

GTX130586 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody (GTX130586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hung YH et al. J Pers Med 2024; 14 (3) Chromatin Remodeling-Related PRDM1 Increases Stomach Cancer Proliferation and Is Counteracted by Bromodomain Inhibitor.
YH Hung et al. J Pers Med 2024; 14 (3) : 224 Chromatin Remodeling-Related PRDM1 Increases Stomach Cancer Proliferation and Is Counteracted by Bromodomain Inhibitor
Application : ICC/IF
Yesbolatova A et al. Nat Commun 2020; 11 (1) : 5701 The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.
Application : WB
Reactivity : Human
Liong S et al. Reproduction 2018; 155 (6) : 573-582 Bromodomain protein BRD4 is increased in human placentas from women with early-onset preeclampsia.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

BRD4 antibody Cat. No. GTX130586

Rating
( Average 4 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Anti-BRD4 antibody used in Western Blot (WB). GTX130586
Date : Anonymous submitted on 25-Apr-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : BT549 cell lysates with/without drug treatment
Amount used : 70μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-BRD4 antibody used in Western Blot (WB). GTX130586
Date : Anonymous submitted on 25-Apr-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cell lysates with/without drug treatment
Amount used : 70μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169