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BRD7 antibody [C1C3]

Anti-BRD7 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118755
Anti-BRD7 antibody [C1C3] used in Western Blot (WB). GTX118755

Cat No. GTX118755

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BRD7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165619

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

bromodomain containing 7 , BP75 , CELTIX1 , NAG4

Cellular Localization

Isoform 2: Nucleus

Background

This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BRD7 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118755

GTX118755 ICC/IF Image

BRD7 antibody [C1C3] detects BRD7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRD7 protein stained by BRD7 antibody [C1C3] (GTX118755) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by () diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-BRD7 antibody [C1C3] used in Western Blot (WB). GTX118755

GTX118755 WB Image

BRD7 antibody detects BRD7 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BRD7 antibody (GTX118755) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169