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BRD9 antibody [HL1282]

Recombinant antibody Orthogonal
Anti-BRD9 antibody [HL1282] used in IHC (Paraffin sections) (IHC-P). GTX636696
Anti-BRD9 antibody [HL1282] used in IHC (Paraffin sections) (IHC-P). GTX636696
Anti-BRD9 antibody [HL1282] used in Western Blot (WB). GTX636696
Anti-BRD9 antibody [HL1282] used in Western Blot (WB). GTX636696
Anti-BRD9 antibody [HL1282] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636696
Anti-BRD9 antibody [HL1282] used in IHC (Paraffin sections) (IHC-P). GTX636696

Cat. No. GTX636696

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1282

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BRD9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BRD9. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910047

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bromodomain containing 9 , LAVS3040 , PRO9856

Database

Research Area

DATA IMAGES

Anti-BRD9 antibody [HL1282] used in IHC (Paraffin sections) (IHC-P). GTX636696

GTX636696 IHC-P Image

BRD9 antibody [HL1282] detects BRD9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
BRD9 stained by BRD9 antibody [HL1282] (GTX636696) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BRD9 antibody [HL1282] used in IHC (Paraffin sections) (IHC-P). GTX636696

GTX636696 IHC-P Image

BRD9 antibody [HL1282] detects BRD9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
BRD9 stained by BRD9 antibody [HL1282] (GTX636696) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BRD9 antibody [HL1282] used in Western Blot (WB). GTX636696

GTX636696 WB Image

Orthogonal

K562 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BRD9 antibody [HL1282] (GTX636696) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD9 antibody [HL1282] used in Western Blot (WB). GTX636696

GTX636696 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BRD9 antibody [HL1282] (GTX636696) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD9 antibody [HL1282] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636696

GTX636696 ICC/IF Image

BRD9 antibody [HL1282] detects BRD9 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRD9 stained by BRD9 antibody [HL1282] (GTX636696) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-BRD9 antibody [HL1282] used in IHC (Paraffin sections) (IHC-P). GTX636696

GTX636696 IHC-P Image

BRD9 antibody [HL1282] detects BRD9 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BRD9 stained by BRD9 antibody [HL1282] (GTX636696) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations