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BRD9 antibody

Anti-BRD9 antibody used in Western Blot (WB). GTX121386
Anti-BRD9 antibody used in IHC (Paraffin sections) (IHC-P). GTX121386
Anti-BRD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121386

Cat No. GTX121386

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BRD9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169683

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

bromodomain containing 9 , LAVS3040 , PRO9856

Database

Research Area

DATA IMAGES

Anti-BRD9 antibody used in Western Blot (WB). GTX121386

GTX121386 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BRD9 antibody (GTX121386) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BRD9 antibody used in IHC (Paraffin sections) (IHC-P). GTX121386

GTX121386 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using BRD9(GTX121386) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BRD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121386

GTX121386 ICC/IF Image

BRD9 antibody detects BRD9 protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRD9 stained by BRD9 antibody (GTX121386) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169